Journal: BMC Infectious Diseases

Article Title: Re-emergence of tularemia in Germany: Presence of Francisella tularensis in different rodent species in endemic areas

doi: 10.1186/1471-2334-8-157

Figure Lengend Snippet: Study sites, Germany, 2004–2006 . Green dots indicate the investigation areas, red dots the three largest German cities for orientation. The number of small mammals caught and the F. tularensis detection rate are shown in parenthesis.

Article Snippet: An aliquot of 20 μl was then lysed with 180 μl of lysis buffer containing proteinase K as previously described [ ] and 5 μl were tested in duplicate using a real-time-PCR for the presence of the 16S rRNA-gene of F. tularensis (LightMix ® -Kit Francisella tularensis , TIB MOLBIOL, Berlin, Germany).

Techniques:

Journal: BMC Infectious Diseases

Article Title: Re-emergence of tularemia in Germany: Presence of Francisella tularensis in different rodent species in endemic areas

doi: 10.1186/1471-2334-8-157

Figure Lengend Snippet: Distribution of rodent trapping areas (marked in green) and water sample collection sites (red dots) in an exemplary area (DA) . The large red dot indicates the water sample tested positive for F. tularensis .

Article Snippet: An aliquot of 20 μl was then lysed with 180 μl of lysis buffer containing proteinase K as previously described [ ] and 5 μl were tested in duplicate using a real-time-PCR for the presence of the 16S rRNA-gene of F. tularensis (LightMix ® -Kit Francisella tularensis , TIB MOLBIOL, Berlin, Germany).

Techniques:

Journal: BMC Infectious Diseases

Article Title: Re-emergence of tularemia in Germany: Presence of Francisella tularensis in different rodent species in endemic areas

doi: 10.1186/1471-2334-8-157

Figure Lengend Snippet: Detection of F. tularensis in different small mammal species

Article Snippet: An aliquot of 20 μl was then lysed with 180 μl of lysis buffer containing proteinase K as previously described [ ] and 5 μl were tested in duplicate using a real-time-PCR for the presence of the 16S rRNA-gene of F. tularensis (LightMix ® -Kit Francisella tularensis , TIB MOLBIOL, Berlin, Germany).

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: PinX1-siRNA/mPEG-PEI-SPION combined with doxorubicin enhances the inhibition of glioma growth

doi: 10.3892/etm.2014.1586

Figure Lengend Snippet: ζ-potential and size of mPEG-PEI-SPION and PinX1-siRNA/mPEG-PEI-SPION (N/P=5).

Article Snippet: Double-stranded oligonucleotides with homology to a desired target region of PinX1 were synthesized by Guangzhou RiboBio Co., Ltd. (Guangzhou, China) and the target sequence was GCTGTGGATCCCAGAAATA.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: PinX1-siRNA/mPEG-PEI-SPION combined with doxorubicin enhances the inhibition of glioma growth

doi: 10.3892/etm.2014.1586

Figure Lengend Snippet: qPCR analysis of PinX1 mRNA expression levels revealed that NC-siRNA did not affect the PinX1 mRNA expression levels (P>0.05, vs. normal group). However, PinX1-siRNA did reduce the PinX1 mRNA expression levels to 57.7% of the NC group ( * P<0.05, vs. normal group). qPCR, quantitative polymerase chain reaction; PinX1, PIN2-interacting protein 1; siRNA, short interfering RNA; NC, negative control.

Article Snippet: Double-stranded oligonucleotides with homology to a desired target region of PinX1 were synthesized by Guangzhou RiboBio Co., Ltd. (Guangzhou, China) and the target sequence was GCTGTGGATCCCAGAAATA.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Small Interfering RNA, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: PinX1-siRNA/mPEG-PEI-SPION combined with doxorubicin enhances the inhibition of glioma growth

doi: 10.3892/etm.2014.1586

Figure Lengend Snippet: Absorbance values of each group using the telomerase PCR ELISA assay. There was no significant difference in the absorbance values between the NC-siRNA and normal groups. However, the PinX1-siRNA group exhibited a markedly decreased absorbance value as compared with the normal group. * P<0.05, vs. normal group. PCR, polymerase chain reaction; PinX1, PIN2-interacting protein 1; siRNA, short interfering RNA; NC, negative control.

Article Snippet: Double-stranded oligonucleotides with homology to a desired target region of PinX1 were synthesized by Guangzhou RiboBio Co., Ltd. (Guangzhou, China) and the target sequence was GCTGTGGATCCCAGAAATA.

Techniques: Enzyme-linked Immunosorbent Assay, Polymerase Chain Reaction, Small Interfering RNA, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: PinX1-siRNA/mPEG-PEI-SPION combined with doxorubicin enhances the inhibition of glioma growth

doi: 10.3892/etm.2014.1586

Figure Lengend Snippet: PinX1-siRNA increased the cell toxicity of DOX. Treatment with 10 μg/ml DOX reduced cell viability to 67% of the normal group. PinX1-siRNA alone did not decrease the cell viability. Cells treated with a combination of PinX1-siRNA and DOX exhibited a cell viability of 54.7%, which was significantly lower when compared with the DOX group. * P<0.05, vs. normal group; # P<0.05, vs. DOX group. DOX, doxorubicin; PinX1, PIN2-interacting protein 1; siRNA, short interfering RNA.

Article Snippet: Double-stranded oligonucleotides with homology to a desired target region of PinX1 were synthesized by Guangzhou RiboBio Co., Ltd. (Guangzhou, China) and the target sequence was GCTGTGGATCCCAGAAATA.

Techniques: Small Interfering RNA

Journal: Experimental and Therapeutic Medicine

Article Title: PinX1-siRNA/mPEG-PEI-SPION combined with doxorubicin enhances the inhibition of glioma growth

doi: 10.3892/etm.2014.1586

Figure Lengend Snippet: Hoechst staining images of C6 cells in the (A) normal control, (B) NC-siRNA control, (C) PinX1-siRNA, (D) DOX and (E) DOX + PinX1-siRNA groups. Representative images revealed that 10 μg/ml DOX caused decreased cell numbers and nucleus fragmentation (arrows). Furthermore, if C6 cells were administered with PinX1-siRNA and DOX simultaneously, cell loss and nuclear condensation (arrows) were markedly more severe. DOX, doxorubicin; PinX1, PIN2-interacting protein 1; siRNA, short interfering RNA; NC, negative control.

Article Snippet: Double-stranded oligonucleotides with homology to a desired target region of PinX1 were synthesized by Guangzhou RiboBio Co., Ltd. (Guangzhou, China) and the target sequence was GCTGTGGATCCCAGAAATA.

Techniques: Staining, Control, Small Interfering RNA, Negative Control